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Research Associate - Conklin Lab

Gladstone Institutes
San Francisco
Closing date
12 Mar 2024

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Contract Type
Category: Science Lab/Area: Conklin Lab Description: Description: The Conklin Lab is seeking a Research Associate I or II, to support research projects aimed at developing CRISPR therapies for neurodegenerative diseases. Project goals include: using iPSCs and iPSC–derived neurons to test CRISPR therapies in patient–derived cells, conducting CRISPRi screens to discover factors that influence gene editing efficiency, and investigating how neuronal DNA repair affects gene editing outcomes. Ideal candidates have strong communication skills, with a preferred background in mammalian cell culture and molecular biology. Applicants interested in progressing to graduate school or medical school within 2–3 years are encouraged to apply. Please submit a resume/CV, unofficial transcript, and cover letter describing your interest in the lab. About the Lab: In the Conklin Lab, we focus on developing gene editing based therapies to treat neurodegenerative diseases. We leverage our expertise in iPSC–based cell models and CRISPR–Cas9 gene editing, to both model genetic diseases and test the effectiveness of gene editing therapies in patient–derived cells. Using state–of–the–art tools to deliver gene editing proteins into human neurons, we have started to identify some of the key barriers making CRISPR editing so difficult in neurons. Now, we are poised to develop cutting–edge strategies to overcome these barriers, hopefully unlocking the full potential of CRISPR editing in clinically relevant cells. In addition to these molecular biology techniques, we are also using computational methods to optimize precise genome editing. For example, we are developing high–throughput methods to find combinations of CRISPR–Cas9 reagents that could optimally treat large and diverse patient populations. These efforts are crucial for making gene editing therapies cost–effective and accessible, to benefit as many people as possible. Duties and Responsibilities: The Research Associate will be directly supervised by a Graduate Student or Postdoctoral Fellow and have regular meetings with the PI, Dr. Bruce Conklin. The main responsibilities of this position will include but are not limited to: Human pluripotent stem cell culture, cell line engineering, and differentiation into neurons. Molecular biology assays including PCRs and next–generation sequencing, to measure editing outcomes. Molecular cloning to generate new plasmid constructs for use in gene editing experiments. Production of virus–like particles and lipid nanoparticles to deliver Cas9 or other gene editing proteins to cells. Maintaining a detailed and thorough lab notebook. Contributing to shared common lab duties, such as maintaining stocks, reagents, and equipment. Routinely presenting findings, in the form of lab meeting presentations. Required Qualifications: BA/BS in molecular biology, cell biology, bioengineering, or a related scientific field. One or more years of experience working/volunteering in a biological research lab. Experience with molecular biology techniques (PCR, molecular cloning, sequencing). Knowledge of scientific equipment (centrifuge, pipettes, thermocyclers, etc.) and basic lab etiquette. Strong communication skills with a history of presenting at scientific group meetings. Attention to detail and meticulous record keeping abilities. Flexibility in schedule; ability to work evenings and weekends when necessary. Familiarity or interest in human genetics/genetic disease and/or CRISPR gene editing. Preferred Qualifications: Experience with human or mammalian cell culture. Experience with CRISPR gene editing, or CRISPR screening. Experience with fluorescence microscopy, next–generation sequencing, flow cytometry, or other biological readouts. Hiring Range: RA I $25/hr $30/hr RA II $66K – $78K Gladstone Perks & Benefits People–work with talented, committed, and supportive teammates within an organization that values each member of its community. A meaningful place to grow and learn–whether it's your professional skills or scientific knowledge, we have the resources and environment to advance either so you can better support Gladstone's mission to drive a new era of discovery in disease–oriented science and to mentor tomorrow's leaders in an inspiring and diverse environment. Healthy work/life balance–you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work. Compensation–competitive salary. Title and salary will be commensurate with education and experience. Excellent benefits–generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation. Gladstone is committed to providing equal employment opportunities to all employees and applicants for employment without regard to race, color, sex, religion, national origin, ancestry, age, marital status, medical condition, physical or mental disability, veteran status, sexual orientation, or any other non–job related characteristic. We make all employment decisions so as to further this principle of equal employment.

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